Details of a person
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Industrial Bio-engineer (Meurice Institute).
Hired on May 2000. Involved in many industrial projects aiming the production of a recombinant protein from the propagation of the microorganism up to the purification as well at lab scale as at pilot scale.
- Host strains: Saccharomyces cerevisiae, Pichia pastoris, E. coli, Lactococcus lactis.
- Fermentation from 2 to 200L (batch / fed-batch, inducible systems)
- Fixed and expanded bed chromatography, ion exchange, hydrophobic interaction or affinity chromatography (IMAC) from 1ml (Aktä) up to 70L resin.
- Pilot control unit (K-Prime)
- Protein analysis by electrophoresis, colorimetry, turbidimetry and HPLC, Western blot, ELISA.
ÄKTA pilot GE Healthcare
Äkta Purifier 100
Cell disrupter Panda
Fermentation Unit 100l
Fermentation Unit 200l
Fermentation Units 10l
Proteins Purification columns
Sorvall centrifuge RC 12B