Molecular Cloning and DNA modification
Molecular Cloning is based on the construction of recombinant DNA, obtained by inserting a genetic fragment of interest into a suitable vector
This strategy includes several key steps:
- DNA cleavage using restriction enzymes
- Ligation of DNA fragments using DNA ligases
- Insertion into a plasmid vector
- Introduction into a host microorganism
Plasmids play a central role as expression vectors. They may contain:
- Antibiotic resistance genes for selection
- Reporter genes for screening
- Multiple cloning sites enabling flexible insertion of DNA fragments
From genetics to bioprocess
Genetic engineering cannot be separated from the downstream stages of industrial development. After the targeted modification of DNA, a strain’s performance depends on its controlled propagation, its controlled fermentation under optimised conditions, the purification of the proteins produced and, finally, their formulation and stabilisation for the final application.
Our expertise in biotechnology and bioprocesses therefore makes it possible to integrate genetic modification into a complete value chain, from the DNA sequence through to the final product ready for industrial valorisation.
Industrial and societal applications
Genetic engineering technologies enable the targeted production of biomolecules of interest such as:
- Agri-food sector
- Biomolecule production
- Environmental bioremediation
- Development of strains capable of producing rare molecules
The production of medicines, vaccines, or enzymes from transgenic microorganisms perfectly illustrates the integration of molecular cloning within an industrial approach.
