Services

Development and scaling-up of protein purification process by chromatography

To complement a recombinant microorganism propagation process producing a protein of interest, we can offer the development of a recovery and purification of this protein until its stabilization by drying. Our laboratory has two working scales that allow either process development (Akta Avant) or scaling up to pilot scales (Akta Pilot). This second scale enables us to work with columns of several tens of liters and to validate the robustness of the developed process. This scale allows, for example, the production of technical batches prior to a transition to a GMP environment. During these steps, we can also develop the assay protocol for the protein of interest.

Downstream processing

Downstream processing (DSP) is a series of unit operations that lead from the harvest of a bioproduct of interest to its formulation, with the aim of purifying and concentrating it. These operations follow bioreactor production and begin with the separation of biomass and supernatant by centrifugation or filtration. Depending on the location of the molecule of interest, it will be necessary to lyse the cells to recover an intracellular protein or in the form of inclusion bodies. Various steps can then follow to purify and concentrate the product, such as purification by chromatography, purification and concentration by ultrafiltration, and buffer exchange by diafiltration. These different steps can be combined to achieve the desired degree of purity. In addition to chromatographic purification operations, our laboratory offers the various steps of a complete downstream processing or any individual step up to stabilization by drying (lyophilization). These operations can be performed on bioproducts resulting from bioreactor propagation or on natural extracts.